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S.Pakalnislogo, V.SitasPlease visit our web site, H.Schneckenburger *, R.RotomskisPlease visit our web site
logo Institute of Physics, A. Gostauto 12, 2600 Vilnius, Lithuania
Please visit our web site Laser Research Centre, Vilnius University, Sauletekio 9, 2040 Vilnius Lithuania
......*
 Institut fur Lasertechnologien in der Medizin und Messtechnik an der Universitat Ulm,
Ulm and Fachhochschule Aalen, Institut fur Angewandte Forschung, Aalen, Germany
Picosecond absorption spectroscopy of biologically active pigments NADH, FMN and fluorescence marker Rhodamine-123
Abstract

Picosecond time-resolved absorption spectroscopy was used to study excited state dynamics of biologically active pigments: reduced nicotinamide adenine dinucleotide (NADH), flavin mononucleotide (FMN) and the mitochondrial marker Rhodamine -123 (Rh-123). The excited state lifetime of the fluorescence marker Rh-123 was about 3.7 ns in aqueous solution. FMN exhibited biexponential transient absorption relaxation kinetics with relaxation lifetimes t1 around 170 ps and t2 around 4.7 ns. The measurements of excitation energy relaxation parameters of the S1 state of NADH are complicated due to formation of transient species and nonexponential behaviour of transient absorption.


 
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