Table 3. Effect of complexation of P2 and P3
to poly(dG-dC)2 and poly(dA-dT)2 on fluorescence lifetimes tf. The numbers in parentheses indicate the relative
amplitudes of the components
| |
R |
tf/ns |
|
R |
tf/ns |
| P2/methanol |
- |
9.7 a) |
P3/methanol |
- |
9.4 b) |
| P2/buffer |
- |
- |
- |
P3/buffer c) |
- |
1.8(0.40) |
11.8(0.60) |
| P2/poly(dA-dT)2 |
0.027 |
3.9(0.95) |
16.6(0.05) |
P3/poly(dA-dT)2 |
0.027 |
2.2(0.76) |
13.1(0.24) |
| P2/poly(dA-dT)2 |
0.071 |
4.2(0.89) |
14.8(0.11) |
P3/poly(dA-dT)2 |
0.053 |
1.9(0.84) |
10.6(0.16) |
| P2/poly(dA-dT)2 |
0.091 |
4.3(0.90) |
14.6(0.10) |
P3/poly(dA-dT)2 |
0.068 |
2.3(0.83) |
12.8(0.17) |
| P2/poly(dG-dC)2 |
0.036 |
3.4(0.96) |
15.1(0.04) |
P3/poly(dG-dC)2 |
0.028 |
1.9(0.87) |
12.1(0.13) |
| P2/poly(dG-dC)2 |
0.105 |
3.5(0.96) |
13.4(0.04) |
P3/poly(dG-dC)2 |
0.086 |
2.1(0.95) |
13.0(0.05) |
a) 1.3 m M P2
b) 2.5 m
M P3
c) ca 2 m
M P3;buffer composition: 20 mM phosphate, pH 7.1,
|